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. Author manuscript; available in PMC: 2019 Mar 27.
Published in final edited form as: Cell Rep. 2019 Jan 29;26(5):1333–1343.e7. doi: 10.1016/j.celrep.2019.01.013

Figure 3. PPARγ-ATMIN Regulation of ATM Signaling Is Conserved in Primary Human Endothelial Cells.

Figure 3.

(A) Representative immunoblots of endogenous nuclear PPARγ interaction with UBR5 in primary pulmonary arterial endothelial cells (PAECs) isolated from controls (Table S5).

(B) Representative immunoblots of HU-induced pATM expression with PPARγ depletion in PAECs.

(C) Representative immunoblots of restoration of HU-induced pATM expression with siRNA (siPPARγ#9)-resistant PPARγ overexpression in human umbilical venous ECs (HUVECs).

(D) Representative immunoblots of HU-induced pATM and pKAP1 with PPARγ or/and ATMIN depletions in PAECs.

(E) Confocal microscopy of PAECs shows effects of PPARγ depletion on pATM foci with hypoxia (<0.1% O2, 24 h) and reoxygenation (10 min). The line in the box of the box and whisker plots marks the median and whiskers correspond to the 10th to 90th percentiles. Unpaired Student t test was used. ****p <0.0001. Scale bars, 20 μm.

siC, siControl; siPγ, siPPARγ. See also Figure S4.