| • Prepare soluble substance for use. |
| • Mix soluble substance with test sample. |
| • Prepare a dilution control containing equal volumes of test sample and an inert substance, such as albumin or saline. |
| • Incubate at optimal temperature and time. |
| • Test the sample plus the substance and the sample plus the inert substance (dilution control) against RBCs that previously gave a positive reaction. |
RBCs = red blood cells.