Figure 11.

Senescence-inducing stimuli inhibit centrosome and mitotic spindle formation. HeLa cells were treated with alisertib (16 μM) for 10 d and H₂O₂ (450 μM) for 2 h and recovered in complete medium for 7 d, or subjected to UV-C radiation (10 J/m²) and cultured in complete medium for 7 d. Cells were also treated with DMSO as a control. Cells were synchronized in mitosis by double thymidine block. Immunofluorescence staining was then performed using antibody probes specific for α-tubulin (green), γ-tubulin (red), and DAPI (blue). Representative images (A) and quantification of cells displaying centrosome formation and a mitotic spindle (B) are shown. Values represent means ± sem. *P < 0.001 (Student’s t test).