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. 2018 Dec 31;33(4):4866–4882. doi: 10.1096/fj.201801382R

Figure 4.

Figure 4

Cellular senescence induced by the down-regulation of caveolin-1 is dependent on primary cilia formation. WI-38 human diploid fibroblasts were transfected with caveolin (Cav)-1 siRNA, together with either IFT88 siRNA or scrambled (Scr) siRNA. Transfection of scrambled siRNA alone was used as the control. Cells were collected for analysis 10 d after transfection. A) Cell lysates were subjected to immunoblot analysis with antibody probes specific for caveolin-1, IFT88, p16, and p21. Immunoblot with anti-β-actin IgGs was performed as the control. B) Primary cilia formation was determined by immunofluorescence analysis with anti-acetylated α-tubulin. Quantification of the staining is shown. C) Cells were subjected to SA-β-gal staining; quantification of the staining is shown. Values represent means ± sem. *P < 0.001 (Student’s t test).