Figure 7.

GABAergic neurons developed in the NEUROG2/1 iN networks. A) Representative iNs immunostained for NeuN (green), GABA (red), and Map2 (purple) on d 30. Scale bar, 100 μm. B) Heatmap of the expression levels of genes encoding inhibitory molecular markers at different days after induction. All expression levels are normalized to those on d 5 (D5). C) Representative raster plots (top) and analysis of the spike rate (bottom) of iN networks recorded by MEA on d 60 before and after PTX (20 μM) treatment. Data were collected from 6 MEAs. **P < 0.01 (unpaired Student’s t test).