Figure 7.

TRB3 knockout maintains protein synthesis rate after 48 h of food deprivation. Frozen TA muscles were processed for analysis. A) Protein synthesis rate was measured by Western blot, and ponceau S staining revealed that an equal amount of proteins was loaded for each sample. B) All puromycin blots were normalized by ponceau S staining. C–F) Protein synthesis pathway factors, including mTOR, 4EBP1, PRAS40, and TSC2, were analyzed by Western blot. FD, food deprivation group; Fed, group with free access to food; A.U., arbitrary unit; p4EBP1^S65, eukaryotic translation initiation factor 4E-binding protein 1; pmTOR^S2448, mechanistic target of rapamycin pPRAS40^T246, proline-rich Akt substrate of 40 kDa; pTSC^T1462, tuberous sclerosis complex 2. Data are the means ± sem (n = 6/group). *P < 0.05, ***P < 0.001 vs. Fed, ^#P < 0.05 vs. WT.