Figure 6.

The importance of residue G.H5.23 in Gα₁₃ sensors is maintained in the full-length G protein sequence. hGPR35-eYFP was coexpressed in HEK293 cells with forms of full-length Gα₁₃ that either were wild type, had residue G.H5.22 converted to Asp, had residue G.H5.23 converted to Ile, or contained both of these alterations. Nanoluc had been introduced into all of the forms of Gα₁₃ to provide a potential BRET pairing with the eYFP-tagged receptor. Cells were then stimulated with varying concentrations of either zaprinast (A) or lodoxamide (B). Data are shown as means ± sd (n = 3).