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. 2019 Jan 22;33(4):5599–5614. doi: 10.1096/fj.201802063RR

Figure 4.

Figure 4

MiR-135a-3p regulates the expression of downstream P38 signaling in ECs. HUVECs transfected with NSm or miR-135a-3pm (A) or miR inhibitor negative control (NSi) or miR-135a-3p inhibitor (miR-135a-3pi) (B), and stimulated with VEGF (50 ng/ml) at the given time points were subjected to Western analysis using antibodies to p-p38, p38, p-ERK1/2, ERK1/2, p-Akt, Akt, and β-actin (n = 3–5 experiments). Ns, nonsignificant. All data represent means ± sem. Differences among groups were analyzed by using 1-way ANOVA. *P < 0.05, **P < 0.005, ***P < 0.001 compared to controls.