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. 2019 Jan 22;33(4):5599–5614. doi: 10.1096/fj.201802063RR

Figure 6.

Figure 6

SiRNA-mediated knockdown of HIP1 recapitulates miR-135-3p functional effects in ECs. A, B) HUVECs were transfected with siRNA to HIP1 or scrambled control (Ctrl) siRNA. Protein expression was determined by Western analysis under baseline conditions (A) or in response to VEGF (50 ng/ml) treatment (B) using antibodies to HIP1, p-P38, P38, and GAPDH (n = 2 experiments). *P < 0.05, **P < 0.001. C–E) HUVECs were transfected with siRNA to HIP1 or scrambled Ctrl siRNA in the presence of NSm or miR-135a-3pm. EC functional angiogenic assays were performed for scratch assay (C), Boyden transwell migration (D), or proliferation by BrdU assay (E). *P < 0.01. F) HUVECs were transfected with siRNA to p38K or siRNA control in the presence of NSm or miR-135a-3pm and EC scratch assays were performed. Ns, nonsignificant. Results are representative of n = 3 replicates/group. All data represent means ± sem. Differences among groups were analyzed by using 1-way ANOVA. *P < 0.01.