Skip to main content
. 2019 Jan 2;33(4):4780–4789. doi: 10.1096/fj.201801959R

Figure 2.

Figure 2

Effects on second messenger generation and signaling upon acute EP-specific stimulation in HASM cells. A) HASM cultures were grown to near confluence in 24-well plates and treated with indicated agonists for 10 min. cAMP generation was isolated, quantified, and represented as a percentage of 100 µM FSK as described in Materials and Methods. B) Calcium mobilization of primary HASM cells stimulated with indicated agonists measured using Flexstation analysis. C) Representative Western blot analysis of HASM cells stimulated for 10 min with indicated agonist. D) Graphic representation of data from Western blot analyses. 248, ONO-AE-248; 259, ONO-AE1-259-01; 329, ONO-AE1-329; Hist, histamine; Sul, sulprostone. E) Representative Western blot of HASM cultures infected with retrovirus enabling expression of GFP (control) or PKI-GFP selected with puromycin, as described in Materials and Methods. Cultures were plated into 12-well plates and stimulated with indicated agonist for 10 min. All concentrations are given in nM, unless otherwise indicated. Graphical data represent mean ± sem values from 3 to 6 experiments, 2-way ANOVA. *P < 0.05.