Skip to main content
. 2019 Jan 16;33(4):5585–5598. doi: 10.1096/fj.201802056R

Figure 2.

Figure 2

HCA attenuates wound repair by HPMECs and SAECs. Monolayers of HPMECs and SAECs were wounded in an in vitro scratch assay and exposed to normocapnia or HCA for 24 h. A) HCA significantly reduced percentage wound closure by HPMECs at 24 h in a noninflammatory (vehicle) but not inflammatory (cytomix) environment. These findings are corroborated by representative images depicting approximate wound areas at 0 and 24 h (n = 4 per group). B) HCA significantly reduced percentage wound closure by SAECs at 24 h in a noninflammatory (vehicle) but not inflammatory (cytomix) environment. These findings are corroborated by representative images depicting approximate wound areas at 0 and 24 h (n = 5 per group). Images were taken by Zeiss Axiovert 25 CFL microscope. +ve Ctrl, positive control. Box and whisker plots show median, first and third quartiles, and maximum and minimum values. Original magnification, ×20. *P < 0.05, **P < 0.01.