Table 3. Structures of Nod Factor synthesized in the presence or absence of 400 mM mannitol by Rhizobium tropici CIAT 899 and its nodD2 mutant derivative.

NF structurea	CIAT 899b	CIAT 899 in B- mannitolb	nodD2 mutantb	nodD2 mutant in B- mannitolb
III (C16:0)	-	+*	-	-
III (C16:0, NMe)	-	+*	-	-
III (C18:0)	-	+	-	-
III (C18:1)	-	+*	-	-
III (C18:1, NMe)	-	+*	-	-
III-Hex (C18:1)	-	+	-	-
III-Hex (C18:1, NMe)	-	+	-	-
IV (C14:0)	-	+*	-	-
IV (C14:0, NMe)	-	+*	-	-
IV (C16:0)	-	+*	-	-
IV (C16:0, NMe)	-	+*	-	-
IV (C16:1)	-	+*	-	-
IV (C18:0)	-	+*	-	-
IV (C18:0, NMe)	-	+*	-	-
IV (C18:1)	+	+*	+	+
IV (C18:1, NMe)	-	+*	-	-
IV (C18:1, NMe, S)	-	+*	-	-
IV (C20:1)	-	+	-	-
IV-Hex (C16:0, NMe)	-	+*	-	-
IV-Hex (C18:0)	-	+	-	-
IV-Hex (C18:0, NMe)	-	+	-	-
IV-Hex (C18:1)	-	+*	+	+
V (C16:0)	-	+*	-	-
V (C18:1, NMe) dNAcc	+	+	-	-
V (C18:1, Cb) dNAcc	-	+	-	-
V (C18:1, NMe) dNAcd	+	+	-	-
V (C16:0, NMe)	-	+*	-	-
V (C18:1)	+	+*	+	+
V (C18:0)	-	+*	-	-
V (C18:0, S)	-	+	-	-
V (C18:1, NMe)	+	+*	+	+
V (C18:1, S)	-	+*	-	-
V (C18:1, NMe, S)	+	+*	-	-
V (C18:0, NMe, S)	-	+*	-	-
V (C20:1, NMe)	-	+	-	-
V (C20:1, NMe, S)	-	+*	-	-
Total	6	36 (25*)	4	4

a. NF structures are represented following the convention in Spaink, 1992 [48] that indicates the number of GlcNAc residues in the backbone (Roman numeral), the length and degree of unsaturation of the fatty acyl chain, and the other substituents, which are listed in the order in which they appear, moving clockwise from the fatty acid. Hex, Hexose; NMe, N-methyl group at glucosamine non-reducing residue; S, sulfate group at reducing glucosamine residue; Cb, carbamoyl group.

b. Symbol: + = detected;— = not detected.

c. Nod Factor deacetylated at glucosamine residue number 3.

d. Nod Factor deacetylated at glucosamine residue number 4.

* NF also synthetized in the presence of salt [10, 11].