. Author manuscript; available in PMC: 2019 Sep 1.

Published in final edited form as: Mucosal Immunol. 2019 Jan 21;12(2):299–311. doi: 10.1038/s41385-018-0130-4

Table 1. Tools to explore ILC2 function.

Location	Cell surface markers	Stimuli	Gene expression or reporter
Lung and gut, skin, lymph nodes, bone marrow, adipose tissue	Lin-, CD25, CD69, CD90 (Thy1), CD127, T1/ST2 (IL-33R), ICOS, KLRG1, Sca-1, IL-17BR (IL-25R) CD117 (c-Kit)	IL-2 IL-7 IL-25 IL-33 TSLP NmU	Rora, Gata3, Il-4 reporter Il-5 reporter Il-9 reporter Il-13 reporter
*Engineered mice targeting ILC2*
Mouse line	Deleted cells	Explanation	References
Rora^sg/fl Il7R^Cre	ILC2		176
Rora^sg/sg	Stagger mouse, deficient in Rora	Rora required for ILC2 development	2
inducible ICOS-diphtheria toxin receptor (iCOS-T)	Temporal deletion of ILC2 with diphtheria toxoid		176
RAG-deficient R5/R5 deleter	IL-5 reporter allele (tdTomato) for identification of ILC2s in adult mice		92 177
RAG-deficient	Deletion of T and B lymphocytes	CD90.2 antibody mediate deletion of ILCs	5
Tcf7^-/-	Deletion of Tcf7 in entire hemaotopoietic compartment	Tcf7 required for ILC2 generation	²⁹ ²⁸
Bcl11b^-/- and Bcl11b^fl/fl ERT2^Cre	Global Bcl11b deficiency or temporal deletion with tamoxifen	Bcl11b required for ILC2 development	178
*Common models and tools used to study ILC2 responses in the lung*
Models/tools	Key outputs		References
House dust mite, Papain, Alternaria, Nippostrongylus	All induce robust type 2 immune responses in the lung with a strong contribution from ILC2s		2 22, 179–182