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. 2019 Mar 27;14(3):e0211134. doi: 10.1371/journal.pone.0211134

Fig 2. KSR1 inhibition attenuates FA-stimulated NT secretion.

Fig 2

(a) BON cells were transfected with KSR1-targeting siRNA or KSR1-targeting shRNA (b) and treated with or without DHA for 90 min. Total RNA was isolated and qRT-PCR performed targeting KSR1 with GAPDH as the internal control (left) (†p<0.05 vs. untreated NTC; *p<0.05 vs. NTC plus DHA). Media were collected and NT-EIA performed (right) (†p<0.05 vs. untreated NTC; *p<0.05 vs. NTC plus DHA; ‡p<0.05 vs. untreated KSR1-siRNA/shRNA groups.) (c) QGP-1 cells were transfected with KSR1-targeting siRNA and treated with or without DHA for 90 min. Total RNA was isolated and qRT-PCR performed targeting KSR1 with GAPDH as the internal control (left) (†p<0.05 vs. untreated NTC; *p<0.05 vs. NTC plus DHA). Media were collected and NT-EIA performed (right) (†p<0.05 vs. untreated NTC; *p<0.05 vs. NTC plus DHA; ‡p<0.05 vs. untreated KSR1-siRNA group). All data represent mean +/- SD. Data are representative of three independent experiments.