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. 2019 Mar 27;14(3):e0211134. doi: 10.1371/journal.pone.0211134

Fig 4. Overexpression of KSR1 enhances NT secretion and ERK activation.

Fig 4

(a) BON cells were transfected with pEGFP-control and pECFP-N-KSR1 plasmid DNA and QGP-1 cells were transfected with MSCV-IRES-GFP and MSCV-KSR1-IRES-GFP plasmid DNA (b) and treated with or without 100μM DHA for 90 min. Cells were lysed and western blotting analysis performed. Band intensity is indicated below the corresponding band and expressed as fold-change relative to NTC. Each blot is representative of two independent experiments. (c) BON cells were transfected with pEGFP-control and pECFP-N-KSR1 plasmid DNA and QGP-1 cells were transfected with MSCV-IRES-GFP and MSCV-KSR1-IRES-GFP plasmid DNA (d) and treated with or without 100μM DHA for 90 min. Media were collected and NT-EIA performed. Panel (c): †p<0.05 vs. untreated pEGFP; *p<0.05 vs. pEGFP plus DHA; ‡p<0.05 vs. untreated CFP-KSR1; Panel (d): †p<0.05 vs. untreated MSCV-GFP; *p<0.05 vs. MSCV-GFP plus DHA; ‡p<0.05 vs. untreated MSCV-GFP-KSR1. Data represent mean +/- SD and are representative of three independent experiments.