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. 2019 Mar 27;14(3):e0211134. doi: 10.1371/journal.pone.0211134

Fig 5. ERK1/2 inhibition attenuates Exo70 expression.

Fig 5

(a) BON cells stably expressing NTC or ERK2 shRNAs were treated with or without 30μM DHA for 90 min. Cells were lysed and western blotting analysis performed. Band intensity is indicated below the corresponding band and expressed as fold-change relative to NTC. (b) Media were collected and NT-EIA performed (†p<0.05 vs. untreated NTC; *p<0.05 vs. NTC plus DHA; data represent mean +/- SD). (c) BON cells pre-treated with DMSO or PD 0325901 for 30 minutes were treated with or without 100μM DHA for 90 min. Cells were lysed and western blotting analysis performed. Band intensity is indicated below the corresponding band and expressed as fold-change relative to NTC. (d) Media were collected and NT-EIA performed (†p<0.05 vs. DMSO without DHA; *p<0.05 vs. DMSO plus DHA; ‡p<0.05 vs. PD without DHA; data represent mean +/- SD). All data are representative of three independent experiments.