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. Author manuscript; available in PMC: 2019 Aug 15.
Published in final edited form as: J Immunol. 2019 Jan 9;202(4):1186–1199. doi: 10.4049/jimmunol.1800504

Figure 3.

Figure 3

TIM4+ macrophage association with apoptotic B cells in bursa of Fabricius. Cryostat bursal sections (10 µm) from 3 week old birds (A) or embryos at day 18 (B) were co-stained for Bu-1 (green) and TIM4 (red) mAbs. The apoptotic B cell (Bu-1+) engulfed by TIM4 macrophages are indicated by arrows. Bursal sections from three week old birds (c) or embryos at day 18 (d) were also analysed by TUNEL to identify apoptotic cells (green) associated with TIM4+ macrophages (red). The nuclei were counterstained with DAPI (blue). The results show representative staining of three serial sections from at least three birds or embryos.