Fig 2. Tissue damage induced by harmonine.

S. mansoni couples were cultured for 72 h with 5 μM harmonine with a change of medium and compound every 24 h. (A, B) Bubble formation (arrows) on the tegument surface in a representative female (A) and male (B) worm. (C) Gut dilatation (arrow) in a female worm, visualized by bright-field microscopy. (D, E) CLSM images of carmine red-stained worms showing the gut lumen of a control male (D) and the dilated lumen (arrow) of a harmonine-treated male (E). Scale bars are 100 μm (A-C) or 50 μm (D, E).