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. 2019 Mar 14;13(3):e0007240. doi: 10.1371/journal.pntd.0007240

Fig 3. AChE transcript levels in S. mansoni and inhibition of enzymatic activity by harmonine.

Fig 3

(A, B) Expression of SmAChE1 (Smp_154600) and SmAChE2 (Smp_125350) in different sexes and mating-states as determined by qPCR, expressed as relative expression vs. the geometric mean of three reference genes (x100 as an arbitrary factor). A summary of three experiments with SEM is shown. (C, D) Enzymatic activity present in protein lysates of male and female worms in the paired or unpaired state given as change of absorbance over time of one representative of two experiments (C) and as summary of two experiments (absorbance at 30 min) (D). Enzymatic activity was determined by Ellman’s method by quantifying absorbance of 5-thio-2-nitrobenzoic acid, which is equivalent to the amount of thiocholine produced from the hydrolysis of acetylthiocholine by AChE. Significant differences as determined by the unpaired t-test are indicated with * p<0.05 or *** p<0.001. M, male; F, female; bs, bisex (paired); ss, single-sex (unpaired).