Table 1. Bacterial strains used in this study.
Strain | Relevant characteristic(s)a | Source or referenceb |
---|---|---|
K. pneumoniae | ||
ATCC13883 | Klebsiella pneumoniae, ATCC 13883 | ATCC |
ATCCΔOmpK35 | OmpK35 deletion strain of ATCC 13883; Tetr | TS |
ATCCOmpK36GD | OmpK36 L3 GD strain of ATCC 13883 | TS |
ATCCΔOmpK36 | OmpK36 deletion strain of ATCC 13883; Kmr | TS |
ATCCΔOmpK35OmpK36GD | OmpK35 deletion strain with OmpK36 L3 GD; Tetr | TS |
ATCCΔOmpK35ΔOmpK36 | OmpK35 and OmpK36 deletion strain of ATCC 13883; Tetr:Kmr | TS |
10.85 | Wild-type Klebsiella pneumoniae, clinical isolate | [21] |
10.85ΔOmpK35 | OmpK35 deletion strain of 10.85; Tetr | TS |
10.85OmpK36GD | OmpK36 L3 GD strain of ATCC 13883 | TS |
10.85ΔOmpK36 | OmpK36 deletion strain of ATCC 13883; Kmr | TS |
10.85ΔOmpK35OmpK36GD | OmpK35 deletion strain with OmpK36 L3 GD; Tetr | TS |
10.85ΔOmpK35ΔOmpK36 | OmpK35 and OmpK36 deletion strain of ATCC 13883; Tetr:Kmr | TS |
10.85ΔOmpK35ΔOmpK36+ pACYC184 | OmpK35 and OmpK36 double deletion mutant of the 10.85 clinical strain with the pACYC184 control plasmid; Tetr:Kmr | TS |
10.85ΔOmpK35ΔOmpK36+ pJIQQ-1 | OmpK35 and OmpK36 double deletion mutant of the 10.85 clinical strain complemented with ompK36; Tetr:Kmr | TS |
10.85ΔOmpK35ΔOmpK36+ pJIQQ-2 | OmpK35 and OmpK36 double deletion mutant of the 10.85 clinical strain complemented with ompK36GD; Tetr:Kmr | TS |
11.76 | Wild-type Klebsiella pneumoniae, clinical isolate | [21] |
11.76ΔOmpK35 | OmpK35 deletion strain of ATCC 13883; Tetr | TS |
11.76OmpK36GD | OmpK36 L3 GD strain of ATCC 13883 | TS |
11.76ΔOmpK36 | OmpK36 deletion strain of ATCC 13883; Kmr | TS |
11.76ΔOmpK35OmpK36GD | OmpK35 deletion strain with OmpK36 L3 GD; Tetr | TS |
11.76ΔOmpK35ΔOmpK36 | OmpK35 and OmpK36 deletion strain of ATCC 13883; Tetr:Kmr | TS |
JIE1333 | OmpK36 L3 GD K. pneumoniae, clinical isolate | [21] |
JIE1334 | OmpK36 L3 GD K. pneumoniae, clinical isolate | [21] |
JIE1335 | OmpK36 L3 GD K. pneumoniae, clinical isolate | [21] |
JIE1348 | OmpK36 L3 GD K. pneumoniae, clinical isolate | [21] |
JIE1383 | OmpK36 L3 GD K. pneumoniae, clinical isolate | [21] |
JIE1462 | OmpK36 L3 GD K. pneumoniae, clinical isolate | [21] |
JIE1474 | OmpK36 L3 GD K. pneumoniae, clinical isolate | [21] |
JIE1482 | OmpK36 L3 GD K. pneumoniae, clinical isolate | [21] |
JIE2038 | OmpK36 L3 GD K. pneumoniae, clinical isolate | [21] |
JIE2055 | OmpK36 L3 GD K. pneumoniae, clinical isolate | [21] |
JIE2218 | OmpK36 L3 GD K. pneumoniae, clinical isolate | [21] |
JIE4101 | OmpK36 L3 TD K. pneumoniae, clinical isolate | WH |
JIE4111 | OmpK36 L3 TD K. pneumoniae, clinical isolate | WH |
JIE4212 | OmpK36 L3 GD K. pneumoniae, clinical isolate | WH |
JIE4609 | OmpK36 L3 TD K. pneumoniae, clinical isolate | WH |
JIE4656 | OmpK36 L3 TD K. pneumoniae, clinical isolate | WH |
JIE4735 | OmpK36 L3 TD K. pneumoniae, clinical isolate | WH |
JIE2771 | OmpK35/OmpK36 deletion K. pneumoniae, clinical isolate. | [22] |
JIE2771 + pACYC184 | K. pneumoniae JIE2771 harbouring pACYC184 | TS |
JIE2771+pJIQQ-1 | K. pneumoniae JIE2771 complemented with ompK36 | TS |
JIE2771+pJIQQ-2 | K. pneumoniae JIE2771 complemented with ompK36GD | TS |
E. coli | ||
DH5α | hsdR17 recAl; high efficiency transformation strain | [23] |
S17 λpir | λpir lysogen of S17 (Tpr Smr thi pro ΔhsdR hsdM+ recA RP4::2-Tc::Mu-km::Tn7) | [24] |
aL3 GD: Gly+Asp duplication in loop 3, ATCC, American Type Culture collection, Tetr; Tetracycline resistant, Kmr; Kanamycin resistant
bTS, This study. WH, Westmead Hospital.