Fig 5. Segmentation of cells subject to varying levels of pheromone treatment.

(A-E) First column shows the phase images of cln1 cln2 cln3 cells without α-factor (A) and with varying levels of α-factor treatment (B-E). Note that the shapes get progressively more irregular as the concentration of the α-factor increases. Second column shows the histogram of percent area gain by distributing the overlapping segmentation regions. Note that histograms are capped at 10%. Third column shows the relationship between size of the cell and the percent cell area gain. The cell sizes are grouped in 100-pixel increments. The average size of each group is plotted against the average percent size gain in that group. The error bars show the standard error of the mean. Note that for small cells area gain percentage is higher than that for larger cells.