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. Author manuscript; available in PMC: 2019 Nov 26.
Published in final edited form as: J Vis Exp. 2018 Nov 26;(141):10.3791/58687. doi: 10.3791/58687

Figure 1: Reprogramming of human fibroblasts to induced pluripotent stem cells (iPSCs).

Figure 1:

A schematic protocol for the reprogramming of human fibroblasts is presented. Fibroblasts are first passaged at a low density into a well of a 6-well format dish, followed by seven transfections performed at 48 h intervals. Medium is replaced 16–20 h after each transfection. Reprogrammed iPSCs are first passaged at approximately day 18 and clonal colonies are picked by day 26. Typically, fibroblast-derived iPSC lines can be frozen down for long-term storage by day 38.