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. Author manuscript; available in PMC: 2019 Nov 26.
Published in final edited form as: J Vis Exp. 2018 Nov 26;(141):10.3791/58687. doi: 10.3791/58687

Figure 3: Colony formation following transfections with reprogramming modified mRNAs and miRNA mimics.

Figure 3:

Low-magnification images were taken of a representative reprogramming on days 15–17. Following the final transfection, reprogrammed iPSCs will form clear colonies with defined boundaries that expand in size and condense to become clearly distinct from incompletely reprogrammed surrounding fibroblasts. Immunostaining for the pluripotency marker TRA-1–60 indicates the presence of iPSCs (inset A) and can be used for calculation of reprogramming efficiency by counting all colonies within a single well (inset B, examples of countable colonies circled in green). Scale bar = 1 mm.