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. Author manuscript; available in PMC: 2019 Nov 26.
Published in final edited form as: J Vis Exp. 2018 Nov 26;(141):10.3791/58687. doi: 10.3791/58687

Figure 4: Representative images of sub-optimal plating density for reprogramming.

Figure 4:

(A, B) Examples of fibroblasts that are too sparse by day 14 of reprogramming (compare to Figure 2, day 14). (C) Low magnification image on day 17 of reprogramming with a large, barren patch circled in red. (D) The same well was fixed and stained for TRA-1–60 to confirm overall poor reprogramming efficiency due to low cell density. Scale bar = 200 μm (A, B) and 1 mm (C, D).