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. Author manuscript; available in PMC: 2019 Mar 27.
Published in final edited form as: Cell Rep. 2018 May 1;23(5):1259–1274. doi: 10.1016/j.celrep.2018.03.126

Figure 2. Distribution of Scaffold Proteins Syd-1 and BRP and the Unc13 Isoforms A and B in the Mushroom Body Calyx of Adult Drosophila.

Figure 2.

Confocal images of adult Drosophila w1118 mushroom body calyces. All scale bars, 10 μm.

(A) Staining against BRPNc82 (green) and Syd-1 (magenta); the blow-up highlights microglomerular structures labeled by yellow dashed lines and arrowheads.

(B) False color-coded median BRP/Syd-1 ratio in calyces. The AZs of KCs were highlighted using BRP-short-GFP (BRPS) expressed by 17D-GAL4 and AZs of PNs using Mz19-GAL4. Color gradients visualize the ratio between Syd-1 and BRPNc82.

(C) GFP-positive median ratios were normalized by median ratios of the surrounding calyx. The AZs positive for 17D-GAL4 > BRPS were enriched for BRP in comparison to Mz19-derived AZs; MWU test result: p = 0.00000983, n = 8 for 17D and n = 13 for Mz19.

(D) Calyx stained against BRPNc82 (green), Unc13A (magenta), and Unc13B, blow-up highlights microglomeruli outlined by yellow dashed lines and arrowheads.

(E) Calyx stained against BRPNc82, Syd-1 (green), and Unc13B (magenta), blow-up shows microglomeruli indicated by yellow dashed lines and arrowheads. Graphs as explained for Figure 1.

See also Figure S2.