Figure 4. Transfer of Fas DNR–modified T cells does not result in acquired ALPS.

(A) Experimental design to analyze long-term persistence of WT pmel-1 CD8α⁺ T cells modified with Fas^ΔDD or empty vector control in B6 mice. (B) Representative FACS plots and (C) summary bar graph of the frequency of CD3⁺B220⁺CD4^–CD8α^– lymphocytes in the spleens of sublethally irradiated WT mice that received 5 × 10⁵ bead-purified Thy1.1⁺ pmel-1 T cells modified with Fas^ΔDD DNR or an empty vector control. Age-matched WT mice and Fas-deficient B6-lpr mice served as negative and positive controls, respectively. ***P < 0.001, *P < 0.05, 2-way ANOVA. (D) Representative FACS plots and (E) summary bar graph demonstrating the persistence and surface phenotype of transferred pmel-1 Thy1.1⁺ T cells modified with Fas^ΔDD DNR or an empty vector control. All data shown are representative of 5 independent experiments, each with n = 5–8 mice per cohort. *P < 0.05, 1-way ANOVA.