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. 2019 Mar 25;48(6):864–872.e7. doi: 10.1016/j.devcel.2019.02.002

Figure 1.

Figure 1

Kinetochore Proteins Concentrate in the Dendritic Extensions of Sensory Neurons

(A) Images of in situ-tagged KNL-1::GFP at different stages of embryogenesis. KNL-1 localized to chromosomes in dividing cells (blue boxes) and to non-chromosomal structures (orange boxes; dashed green line). Time is relative to first cleavage. Scale bars, 10 μm and 2 μm (insets).

(B) Kinetochore assembly in the early C. elegans embryo. Proteins labeled in green were in situ GFP-tagged for localization analysis; underlined proteins were subjected to functional analysis. Scale bar, 2 μm.

(C) Localization of indicated in situ-tagged GFP fusions in the anterior of 1.5-fold stage embryos. Scale bars, 10 μm (brightfield) and 2.5 μm (magnified anterior region).

(D) Localization of mScarlet-fused Nuf2HIM-10 and Pdyf-7-controlled GFP::β -tubulin (TBB-2). Scale bar, 5 μm.

(E) Split GFP analysis with indicated complementing pieces. Scale bars, 10 μm and 2.5 μm (inset).