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. 2019 Mar 25;48(6):864–872.e7. doi: 10.1016/j.devcel.2019.02.002

Figure 2.

Figure 2

Post-Mitotic Degradation of KMN Proteins Causes Sensory Nervous System Defects

(A) Approach used to degrade in situ GFP fusions (top). Images below show loss of KNL-1::GFP signal in presence of a Pdyf-7-controlled GFP degrader. Scale bar, 5 μm.

(B) Images of head sensory neuron nuclei and plasma membranes for the indicated conditions. Scale bar, 10 μm.

(C) Quantification of dispersion of sensory neuron cell bodies, measured as indicated with yellow lines in (B). Error bars denote the 95% confidence interval. ∗∗∗∗ and ns indicate p < 0.0001 and not significant, respectively.

(D) Rescue of sensory nervous system defect by transgene-encoded untagged NDC-80. Scale bar, 10 μm.

(E) Profile of ASER neuron (left) and localization of the synaptic marker SNB-1 (right) in the ASER neuron in the indicated conditions. Arrowheads point to ectopic neurites. Scale bar, 10 μm.

(F) Egg-laying defect: example image (top) and quantification for indicated conditions (bottom). Scale bar, 100 μm. Error bars denote the 95% confidence interval. ∗∗∗∗ and ns indicate p < 0.0001 and not significant, respectively.