Skip to main content
. 2019 Mar 25;48(6):864–872.e7. doi: 10.1016/j.devcel.2019.02.002

Figure 3.

Figure 3

KMN Proteins Are Important for Amphid Bundle Dendrite Extension

(A) Schematic relating amphid sensilla in the embryo to their eventual structure in the L1 larva (each sensilla has 12 neurons; only 5 are depicted).

(B) Live imaging of amphid bundle dendrite extension for the indicated conditions using a Pdyf-7 controlled plasma membrane marker. Scale bar, 2.5 μm.

(C) Quantification of amphid bundle dendrite extension rate. Error bars denote the 95% confidence interval. ∗∗∗∗ and ns indicate p < 0.0001 and not significant, respectively.

(D) Color overlay of gamma-tubulin complex (GIP-2::GFP) and KMN protein (Nuf2HIM-10::mSc) localization. Magnified insets with individual channel signals are shown on the right. Scale bars, 10 μm (embryo) and 1 μm (inset). Line profile along dendrite of bottom inset is shown on the right.

(E) EB1EBP-2::mNG dynamics in the amphid bundle. A plus end comet (arrowhead) and a cluster (asterisk) are highlighted in the top still image. Kymograph along the red line in the 60 s time projection image (bottom) is shown on the right. Scale bar, 2.5 μm.