Table 2.
SAXS Data Collection and Derived Parameters
| MbcTA | |
|---|---|
| Data collection | |
| Instrument | P12 at EMBL/DESY, storage ring PETRA III, Germany |
| Beam geometry | 0.2 × 0.12 mm2 |
| Wavelength (Å) | 1.24 |
| q-range (Å−1) | 0.008–0.47 |
| Exposure time (ms) | 20 × 50 |
| Concentration range (mg mL−1) | 0.6–7.1 |
| Temperature (K) | 283 |
| Structural parametersa | |
| I(0) (arbitrary units) (from P(r)) | 31,320 ± 10 |
| Rg (from P(r)) (Å) | 41 ± 1 |
| I(0) (arbitrary units) (from Guinier) | 31,340 ± 30 |
| Rg (Å) (from Guinier) | 41 ± 1 |
| Dmax (Å) | 114 |
| Porod volume (103 Å3) | 262 |
| Molecular mass determinationa | |
| MMPOROD (from Porod volume) (kDa) | 154 ± 15 |
| MMsaxs (from I(0), kDa) | 110 ± 20 |
| MMDAM (from bead model, kDa) | 170 ± 35 |
| Calculated monomeric MM from sequence (kDa) | 197.2 |
| Software employed | |
| Primary data reduction | Automated radial averaging |
| Data processing | PRIMUS |
| Ab initio analysis | DAMMIN |
| Validation and averaging | SASRES, DAMAVER |
| Computation of model intensities | CRYSOL |
| SASBDB entry code | SASDD33 |
a
Reported for MbcTA at 0.6 mg mL−1