Table 2.

EM Data Collection and Processing

	Ysh1-Mpe1-Yjr141w	CPFcore		CPFpol + Cft2
	Cryo-EM	Cryo-EM	Negative-Stain EM	Negative-Stain EM
Data collection
Microscope	Titan Krios	FEI Tecnai Polara	FEI Tecnai Spirit	FEI Tecnai Spirit
Detector	K2	Falcon III	Ultrascan 1000	Ultrascan 1000
Magnification	105,000 ×	59,000 ×	26,000 ×	26,000 ×
Pixel size (Å)	1.09	1.78	3.98	3.98
Voltage (keV)	300	300	120	120
Electron dose (e-/Å2)	∼45	∼60	∼40–60	∼40–60
Defocus range (μm)	−0.5 to −0.7	−2.5 to −4.5	−0.6	−0.6
Phase shift range (°)a	20–140	N/A	N/A	N/A
Number of particles	43,308	120,773	23,969	38,142
Processing
Resolution	4.8	N/Ab	20	N/Ab
Efficiency (Eod)c	0.29	N/Ab	0.79	N/Ab

N/A, not available.

^aVolta phase plate used during data collection.

^b3D reconstruction not performed.

^cNaydenova and Russo (2017).