REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-peroxiredoxin 2 antibody	Abcam	Cat#ab109367; RRID: AB_10862524
Anti-peroxiredoxin 3 antibody	Abcam	Cat#ab73349; RRID: AB_1860862
Anti-glutathione synthetase antibody	Abcam	Cat#ab91591; RRID: AB_2049703
Anti-TOMM20 antibody	Santa Cruz	Cat#sc-11415; RRID: AB_2207533
Goat anti-rabbit IgG H&L (AlexaFluor 594)	ThermoFisher	Cat#A11037; RRID: AB_2534095
Anti-triphenylphosphonium antibody	Lin et al. (2002)
gamma-glutamylcysteine synthetase antibody	ThermoFisher	Cat#PA5-44189; RRID: AB_2608691
IRDye 680LT Goat anti-Mouse IgG	LICOR	Cat#925-68020; RRID: AB_2687826
IRDye 800CW Donkey anti-Rabbit IgG	LICOR	Cat#925-32213; RRID: AB_2715510
PRX pathway Western Blot Cocktail (TRX, TXNRD1, PRX1)	Abcam	Cat#ab184868
RNAseq Data deposited to PubMed Geo under GSE117425	This study	PubMed ID (GSE117425)
Chemicals, Peptides, and Recombinant Proteins
(4-Iodobutyl)triphenylphosphonium (IBTP)	Abcam	Cat#ab144646;CAS#159085-21-9
1-chloro-2,4-dinitrobenzene	Sigma-Aldrich	Cat#237329; CAS#97-00-7
5-sulfosalicylic acid hydrate	Sigma-Aldrich	Cat#390275; CAS#304851-84-1
acivicin	Sigma-Aldrich	Cat#sml0312; CAS#42228-92-2
acrolein	Sigma-Aldrich	Cat#89116; CAS#107-02-8
Alamethicin from Trichoderma viride	Sigma-Aldrich	Cat#A4665; CAS#27061-78-5
Amplex Red	ThermoFisher	Cat#A12222; CAS#119171-73-2
Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone	Sigma-Aldrich	Cat#C2920; CAS#370-86-5
d15-MitoB	Cayman Chemical	Cat#17470
Disodium succinate	Sigma-Aldrich	Cat#w327700; CAS#150-90-3
DTNB	ThermoFisher	Cat#22582; CAS#69-78-3
Glutathione Reductase	Sigma-Aldrich	Cat#10105678001
HRP	ThermoFisher	Cat#31490; CAS#9003-99-0
Human recombinant Peroxiredoxin 1	Abcam	Cat#ab79945
Human recombinant thioredoxin 1	Abcam	Cat#ab51064
Human recombinant TXRND1	Sigma-Aldrich	Cat#SRP6081
Hydrogen peroxide solution	Sigma-Aldrich	Cat#H1009; CAS#7722-84-1
L-glutathione	Sigma-Aldrich	Cat#G4251; CAS#70-18-8
L-glutathione oxidised	Sigma-Aldrich	Cat#G4376; CAS#27025-41-8
Lipofectamine 2000	ThermoFisher	Cat#11668027
Methyltriphenylphosphonium bromide (TPMP)	Sigma-Aldrich	Cat#130079; CAS#1779-49-3
Methyltriphenylphosphonium iodide [methyl-3H]	American Radiolabelled Chemicals	Cat#0724-250
MitoCDNB	this paper	n/a
MitoCysDNB	this paper	n/a
MitoGSDNB	this paper	n/a
MitoPQ	Abcam	Cat#ab146819; CAS#146819-28-8
MitoSOX red	ThermoFisher	Cat#M36008
NADPH Tetrasodium salt	Sigma-Aldrich	Cat#10107824001; CAS#2646-71-1
N-ethylmaleimide (NEM)	Sigma-Aldrich	Cat#E3876; CAS#128-53-0
Propidium Iodide	ThermoFisher	Cat#P1304MP; CAS#25535-16-4
Recombinant Human GSTK1 protein	Abcam	Cat#ab93457
Recombinant Human SOD2	ThermoFisher	Cat#LF-P0013
Rotenone	Sigma-Aldrich	Cat#R8875; CAS#83-79-4
Seleno-L-cystine	Sigma-Aldrich	Cat#545996; CAS#29621-88-3
Succinate	Sigma-Aldrich	Cat#224731; CAS#150-90-3
Critical Commercial Assays
BCA Assay Kit	ThermoFisher	Cat#23225
PureLink RNA Mini Kit	ThermoFisher	Cat#12183018A
Deposited Data
RNASeq Data	This paper	GSE117425
Experimental Models: Cell Lines
HEPG2	ATCC	Cat#HB-8065; RRID: CVCL_0027
C2C12	ATCC	Cat#CRL-1772; RRID: CVCL_0188
Experimental Models: Organisms/Strains
C57BL/6J Mice	Charles River	Cat#664
Wistar Rat	Charles River	Cat#003
Recombinant DNA
pLYS1-FLAG-MitoGFP-HA	Vamsi Mootha Lab (Sancak et al., 2013)	AddGene#50057
Software and Algorithms
GraphPad Prism	ThermoFisher	https://www.thermofisher.com/order/catalog/product/INQSOF012
CASAVA 1.8	Illumina	https://support.illumina.com/sequencing/sequencing_software/bcl2fastq-conversion-software.html
TrimGalore!	Babraham Bioinformatics	http://www.bioinformatics.babraham.ac.uk/projects/trim_galore/
HISat2	John Hopkins Centre for Computational Biology	https://ccb.jhu.edu/software/hisat2/index.shtml
FeatureCounts	Subread, WEHI	http://subread.sourceforge.net/
FlowJo 10.4.2	FlowJo	https://www.flowjo.com/
DESEQ2	Bioconductor	https://bioconductor.org/packages/release/bioc/html/DESeq2.html
ImageStudio Lite	LICOR	https://www.licor.com/bio/products/software/image_studio_lite/
ImageJ	NIH	https://imagej.nih.gov/ij/
Other
96-well polypropylene hydrophilic PVDF 0.45 μm filter plate	Porvair Sciences	Cat#360043
1 x 50 mm MS column	Waters	Cat#186002344