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. Author manuscript; available in PMC: 2019 Mar 27.
Published in final edited form as: Cell Rep. 2019 Mar 5;26(10):2805–2817.e9. doi: 10.1016/j.celrep.2019.02.022

Figure 5. NEX–Trio+/− and NEX–Trio−/− Mice Have Increased Dendritic Spine Density and Smaller Synapses in the Motor Cortex.

Figure 5.

(A and C) Representative basal dendrite segments from L5 PNs in the motor cortex of NEX–Trio+/− (A) and NEX–Trio−/− (C) mice with WT controls. Scale bars represent 5 mm.

(B and D) Dendritic spine density was increased on L5 PNs in the motor cortex of NEX–Trio+/− (B) and NEX–Trio−/− (D) mice relative to WT controls. A linear regression with post hoc Bonf MC test identified differences (n = 17–27 dendrite segments from R3 mice per group).

(E) Representative electron micrographs from L5 motor cortex of NEX–Trio+/−, NEX–Trio−/−, and WT mice. Scale bars represent 0.3 μm.

(F and G) Cortical synapse density (synapses per mm ) trended (p = 0.085) toward an increase in NEX–Trio+/− mice (F) and was increased in NEX–Trio−/− mice (G).

(H and I) PSD length was decreased in L5 motor cortex of both NEX–Trio+/− (H) and NEX–Trio−/− (I) mice.

(J and L) Presynaptic bouton area was decreased in L5 motor cortex of both NEX–Trio+/− (J) and NEX–Trio−/− (L) mice.

(K and M) Dendritic spine area was decreased in both NEX–Trio+/− (K) and NEX–Trio−/− (M) mice. For (F)–(M), a linear regression with post hoc Bonf MC test identified differences (n = 54 fields of view [55 μm2 each] from 3 mice per group).

See also Figure S6. Data are represented as mean ± SEM (**p < 0.01; ***p < 0.001; ****p < 0.0001).