| Antibodies |
| Rabbit Primary Antibody: TRIO Sec14 domain |
Katrancha et al., 2017; McPherson et al., 2005
|
N/A |
| Rabbit Primary Antibody: Phosphodiesterase 4D (PDE4D) |
Millipore |
Cat#ABS22 |
| Rabbit Primary Antibody: Phosphodiesterase 4A (PDE4A) |
Abcam |
Cat#ab14607 |
| Rabbit Primary Antibody: Phosphodiesterase 4A Isoform 5 (PDE4A5) |
Abcam |
Cat#ab42094 |
| Rabbit Primary Antibody: P21 (RAC1) Activated Kinase 4 (PAK4) |
Proteintech |
Cat#14685–1-AP |
| Rabbit Primary Antibody: Clathrin Heavy Chain (CHC) |
Pietro De Camilli lab, Yale University |
N/A |
| Goat Secondary Antibody: Anti-Rabbit |
Bio-Rad |
Cat#170–6515 |
| Nanobody: anti-GFP-booster_Atto488 |
Chromotek |
Cat#gba-488 |
| Mouse Primary Antibody: anti-RFP |
Rockland |
Cat#200–301-379 |
| Rabbit Primary Antibody: TRIO Guanine Nucleotide Exchange Factor (GEF) Dbl Homology 2 (DH2) domain |
Katrancha et al., 2017 |
N/A |
| Donkey Secondary Antibody: Anti-Mouse AlexaFluor568 |
Invitrogen |
Cat#A10037 |
| Donkey Secondary Antibody: Anti-Rabbit AlexaFluor647 |
Invitrogen |
Cat#A31573
|
| Chemicals, Peptides, and Recombinant Proteins |
| Lipofectamine 3000 |
Invitrogen |
Cat#L3000001 |
| Permount |
Fisher Scientific |
Cat#SP15–100 |
| AquaMount |
Lerner Laboratories |
Cat#13800 |
| ProLong Diamond Antifade Mountant |
Invitrogen |
Cat#P36970
|
| Rp-cAMPS |
Tocris |
Cat#1337 |
| Tandem mass tag TMT10 |
Thermo |
Cat#90406; Lot#SE240163 |
| Tandem mass tag TMT11 channel |
Thermo |
Cat#1863196B; Lot#RK245431 |
| Poly-D-lysine |
Corning |
Cat#354210 |
| Human fibronectin |
GIBCO |
Cat#33016–015 |
| Critical Commercial Assays |
| FD Rapid GolgiStain Kit |
FD NeuroTechnologies, Inc |
Cat#PK401 |
| Deposited Data |
| Original mass spectra download under the identifier: < MSV000083386 > |
MassIVE (https://massive.ucsd.edu/ProteoSAFe/static/massive.jsp) |
ftp://massive.ucsd.edu/MSV000083386 |
| Experimental Models: Cell Lines |
|
Trioflox/flox mouse fibroblasts |
This Paper |
N/A |
| Experimental Models: Organisms/Strains |
| Mouse (C57BL/6 × 129/SvJ): “floxed” Trio allele: Trioflox/flox (Trio−/−) |
Zong et al., 2015 |
N/A |
| Mouse (C57BL/6 × 129/SvJ): NEX(Neurod6)-Cre transgene: NEX-Cre
|
Goebbels et al., 2006 |
N/A |
| Mouse (C57BL/6 × 129/SvJ): Thy1-GFP-M line: Thy1-GFP
|
Feng et al., 2000 |
N/A |
| Oligonucleotides |
| “Floxed” Trio genotyping primer 1: 5′-TTG TTC CAT TTA CGT CAC CG-3′ |
Zong et al., 2015 |
N/A |
| “Floxed” Trio genotyping primer 2: 5′-CCT CAG GGA AGA GAC TA-3′ |
Zong et al., 2015 |
N/A |
|
GFP genotyping primer 1: 5′-GCA CGA CTT CAA GTC CGC CAT GCC-3′ |
Omar et al., 2017 |
N/A |
|
GFP genotyping primer 2: 5′-GCG GAT CTT GAA GTT CAC CTT GAT GCC-3′ |
Omar et al., 2017 |
N/A |
|
NEX-Cre genotyping primer 1: 5′-AGA ATG TGG AGT AGG GTG AC-3′ |
Omar et al., 2017 |
N/A |
|
NEX-Cre genotyping primer 2: 5′-GAG TCC TGG AAT CAG TCT TTT TC-3′ |
Omar et al., 2017 |
N/A |
|
NEX-Cre genotyping primer 3: 5′-CCG CAT AAC CAG TGA AAC AG-3′ |
Omar et al., 2017 |
N/A |
| Recombinant DNA |
| pEGFP-N1 |
Clonetech |
N/A |
| pAAV-CAG-“GFP-P2A-Cre” |
Jaime Grutzendler, Yale University |
N/A |
| pTriEx-6-PDE4A5 |
Becky Carlyle and Angus Nairn, Yale University |
N/A |
| pLL3.7-RFP |
Lin et al., 2013 |
N/A |
| pAAV-mCherry-IRES-Cre |
Karl Deisseroth, Stanford University (via Addgene) |
Addgene Plasmid #55632 |
| Software and Algorithms |
| Neurolucida software |
MicroBrightField (MBF) Bioscience |
https://www.mbfbioscience.com/neurolucida |
| NeuroExplorer software |
Nex Technologies |
https://www.neuroexplorer.com/ |
| ImageJ software |
NIH |
https://imagej.nih.gov/ij/ |
| Mini-Analysis software |
Synaptosoft |
http://www.synaptosoft.com/MiniAnalysis/ |
| Spectrum Mill software |
Agilent Technologies |
proteomics.broadinstitute.org/ |
| SR Lab Startle Response System software |
San Diego Instruments |
https://www.sandiegoinstruments.com/product/sr-lab/ |
| AnyMaze software |
Stoelting |
http://www.anymaze.co.uk/ |
| Fusion software, Version 4.5 |
Omnitech Electronics |
http://www.omnitech-electronics.com/product/Fusion-Software/510 |
| Morpheus software |
Broad Institute of MIT and Harvard |
https://software.broadinstitute.org/morpheus/ |
| Phoxtrack tool |
Weidner et al., 2014 |
N/A |
| pClamp software suite (pClamp and Clampfit) |
Molecular Devices |
https://www.moleculardevices.com/products/axon-patch-clamp-system/ |
| N-1 Chi-Square test |
MeasuringU |
https://measuringu.com/ab-cal/ |
| R Studio software |
RStudio |
https://www.rstudio.com/ |
| Prism 7 software |
GraphPad |
https://www.graphpad.com/scientific-software/prism/ |
| Other |
| Elevated Plus Maze |
Stoelting |
Cat#60140 |
| Five-lane rotarod treadmill |
Med Associates |
Cat#ENV-577M |
| Open Field Test |
Omnitech Electronics |
http://www.omnitech-electronics.com/product/Open-Field-Locomotor-Activity/1010 |
| Optimal Cutting Temperature Compound |
Tissue-Tek |
Cat#4583 |
| Cryomold (10 mm × 10 mm × 5 mm) |
Tissue-Tek |
Cat#4565 |
| Colorfrost Plus microscope slides |
Thermo Scientific Shandon |
Cat#9991013 |
| TissueTUBE TT1 |
Covaris |
Tube Cat#520001; Plug Cat#520006 |
| CP02 cryoPREP Automated Dry Pulverizer |
Covaris |
Cat#500001 |
| Axopatch patch-clamp amplifier |
Molecular Devices |
Cat#700B |
