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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Biochim Biophys Acta Biomembr. 2019 Mar 13;1861(5):1030–1036. doi: 10.1016/j.bbamem.2019.03.005

Figure 2. Effect of CaCl2 on ND sample integrity.

Figure 2.

ApoA-I containing PC ND and CL ND were formulated in HEPES buffer as described and aliquots of each (corresponding to 78 nmol CL or 147 nmol PC) were applied to the wells of a 96 well microtiter plate. To each well, indicated amounts of CaCl2 (in HEPES buffer) were added and the volume adjusted to 200 μl with HEPES buffer. The plate was incubated for 1 h at 22 °C and, following incubation, sample absorbance measured at 325 nm on a SpectraMax plater reader. Values reported are the mean ± standard error (n = 9) ****, P<0.0001 versus HEPES control and PC ND.