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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Biochim Biophys Acta Biomembr. 2019 Mar 13;1861(5):1030–1036. doi: 10.1016/j.bbamem.2019.03.005

Figure 3. Effect of alternate cations on ND sample integrity.

Figure 3.

Aliquots of CL ND (78 nmol CL), solubilized in HEPES buffer, were applied to the wells of a 96 well microtiter plate. To each well, specified quantities of the chloride salt of mono- or divalent cations was added and the volume adjusted to 200 μl with HEPES buffer. The plate was incubated for 1 h at 22 °C followed by sample absorbance determination at 325 nm. Values reported are the mean ± standard error (n = 3) ****, P<0.0001 versus 0 nmol divalent cation.