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. Author manuscript; available in PMC: 2020 Mar 27.
Published in final edited form as: J Am Chem Soc. 2019 Mar 14;141(12):4952–4962. doi: 10.1021/jacs.9b00146

Figure 8.

Figure 8.

Dependence of thymine excision activity (log kmax) on nucleotide flipping (log Kflip) for TDG or A145G and G·Txy substrates, at 18 °C. Linear fitting gives a slope of m = 1.03 ± 0.05 (r = 0.996) for TDG (●, black line). Data for A145G-TDG (■) were fitted with or without G·TTT (★). Fitting that excludes G·TTT (solid red line) gives a slope of m = 0.97 ± 0.11 (r = 0.988); fitting that includes G·TTT (dotted red line) gives a slope of m = 1.20 ± 0.23 (r = 0.949). Data fitting and kmax and Kflip values are in Supporting Information Table S3, Figure S3.