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. 2019 Mar 21;10:577. doi: 10.3389/fmicb.2019.00577

FIGURE 4.

FIGURE 4

Identification of sub-cluster 3 as the origin of promyelocytes, activated neutrophils, erythrocytes, megakaryocytes, monocytes, and CMV+ cells. (A) Pseudotime ordering of data from cells belonging to the CL7, erythro, mono, MDDC, CMV, promyelo, act neut and sub-cluster 3 groups shown in Figure 3C into a two-dimensional component space using Monocle. The main path of the minimum spanning tree is depicted by solid black lines arising from a central root of cells with a pseudotime of zero (dark blue dots), and branching outward to clusters with higher pseudotime values, representing differentiated cell types (purple, orange, and yellow dots). (B) Cell group labeling based on the expression of key marker genes identified with Seurat. GR, group; MDDC, monocyte-derived dendritic cells; CL7, CL7 from Figure 3C; GEMM, colony-forming unit-granulocyte, erythrocyte, monocyte/macrophage, megakaryocyte; Mono, monocytes; Erythro, erythrocytes; Act Neut, activated neutrophils; Promyelo, promyelocytes.