The influence of MpkA on the MpkB-GFP nuclear translocation. (A) Conidia were grown for 16 h at 30°C before stress induction. The compounds used and the times of the induced stresses are also reported. The data represent the averages of three repetitions ± standard deviations. The nuclei of about 50 germlings were counted in each repetition (*, P < 0.05; **, P < 0.005; ***, P < 0.001; ****, P < 0.0001). Hoechst counterstaining was used to confirm the nuclear localization of the GFP signal. (B) The wild-type, ΔmpkA, and ΔmpkB strains were grown on MM for 5 days at 37 and 44°C, respectively.