TABLE 1.
A. fumigatus strains used in this study
| Description | Target gene | Relevant genotypea | Reference or source |
|---|---|---|---|
| Wild type | CEA10 | 62 | |
| ΔakuB (recipient strain) | AFUB_019720 | CEA17 akuB::pyrG; PyrG+ | 63 |
| ΔmpkA | AFUB_070630 | ΔakuB mpkA::ptrA; PTR | 36 |
| mpkAc (complemented ΔmpkA strain) | ΔakuB ΔmpkA ΔmpkA::mpkA-hph; HygR PTR | 36 | |
| ΔmpkB | AFUB_078810 | ΔakuB mpkB::ptrA; PTR | This study |
| ΔmpkB::mpkB+ | ΔakuB ΔmpkB ΔmpkB::mpkB-hph; HygR | This study | |
| ΔpksP | AFUB_033290 | ΔakuB pksP::hph; HygR | 35 |
| ΔmpkB ΔpksP | ΔakuB ΔmpkB pksP::hph; HygR PTR | This study | |
| ΔmpkA ΔmpkB | ΔakuB ΔmpkB mpkA::ptrA; HygR PTR | This study | |
| ΔgpaA | AFUB_012620 | ΔakuB gpaA::ptrA; PTR | This study |
| gpaAc | ΔakuB ΔgpaA gpaA-hph; HygR PTR | This study | |
| ΔgpaA ΔpksP | ΔakuB ΔgpaA pksP::hph; HygR PTR | This study | |
| ΔgpaB | AFUB_012410 | ATCC 46645 gpaB::hph; HygR | 39 |
| ΔgpaC | AFUB_036760 | ΔakuB gpaC::ptrA; PTR | This study |
| ΔgprM | AFUB_090880 | ΔakuB gprM::ptrA; PTR | This study |
| gprMc | ΔakuB ΔgprM ΔgprM::gprM-hph; HygR | This study | |
| ΔgprM ΔpksP | ΔakuB ΔgprM pksP::hph; HygR PTR | This study | |
| mpkB-gfp | ΔakuB mpkB::mpkB-gfp-hph; HygR | This study | |
| ΔmpkA mpkB-gfp | ΔakuB mpkB-gfp mpkA::ptrA; HygR PTR | This study | |
| gpaA-gfp gprM-3×HA | CEA10 gpaA-gfp gprM-3×HA; PTR | This study | |
| gfp gprM-3×HA | CEA10 gfp gprM-3×HA; PTR | This study | |
| ΔhmgA | AFUB_021290 | ΔakuB hmgA::ptrA; PTR | 32 |
| ΔhppD | AFUB_021270 | ΔakuB hppD::ptrA; PTR | 32 |
| ΔgprA | AFUB_034900 | ΔakuB gprA::hph; HygR | This study |
| ΔgprB | AFUB_055410 | ΔakuB gprB::hph; HygR | This study |
| ΔgprC | AFUB_090350 | ΔakuB gprC::hph; HygR | This study |
| ΔgprD | AFUB_028290 | ΔakuB gprD::hph; HygR | This study |
| ΔgprH | AFUB_052640 | ΔakuB gprH::hph; HygR | This study |
| ΔgprI | AFUB_047630 | ΔakuB gprI::hph; HygR | This study |
| ΔgprJ | AFUB_007220 | ΔakuB gprJ::hph; HygR | This study |
| ΔgprK | AFUB_101830 | ΔakuB gprK::hph; HygR | This study |
| ΔgprO | AFUB_038590 | ΔakuB gprO::hph; HygR | This study |
| ΔgprP | AFUB_073100 | ΔakuB gprP::hph; HygR | This study |
| ΔnopA | AFUB_088000 | ΔakuB nopA::hph; HygR | This study |
a
HygR, hygromycin; PTR, pyrithiamine.