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Primers used
| Primer | Sequence |
|---|---|
| PF ACA 20up | GGCGTATTTTGACACCTAACGAGG |
| PR ACA 20up | CCTCGTTAGGTGTCAAAATACGCC |
| PF ACA 40up | CTCGTTGGAGACATTCATGGCGT |
| PR ACA 40up | ACGCCATGAATGTCTCCAACGAG |
| PF ACA 80up | ATATTGAGCAGACACCCCGGTGAAG |
| PR ACA 80up | CTTCACCGGGGTGTCTGCTCAATAT |
| PF ACA 100up | CGAAGATATCGACAGAGTTAATAT |
| PR ACA 100up | ATATTAACTCTGTCGATATCTTCG |
| PF ACA 120up | GGGGTGCTCGACATAAGCCGAAG |
| PR ACA 120up | CTTCGGCTTATGTCGAGCACCCC |
