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. 2019 Mar 21;10:534. doi: 10.3389/fimmu.2019.00534

Figure 2.

Figure 2

MBCs are acutely dependent on VLA-4 for their retention in the spleen with an additive requirement of LFA-1 (A–D). Flow cytometric analysis of PB taken from SLC−/− mice after 5 h treatment with anti-LFA-1 and/or anti-α4 antibodies (A) Numbers of leukocytes (B) Numbers of MBCs (C) Numbers of MZ B cells (D) %MBCs as proportions of mature B cells (left panel), % of MZ B cells as proportions of mature B cells (middle panel) and ratio of MBC:MZ expressed as proportions of mature B cells (right panel) (E). Flow cytometric analysis of spleen cells: %MBCs as proportions of mature B cells (left panel), % of MZ B cells as proportions of mature B cells (middle panel) and ratio of MBC:MZ expressed as proportions of mature B cells (right panel). n = 4 (treated) and n = 3 (isotype control); error bars show mean +/–SD; data are representative of two independent experiments. One-way ANOVA with Tukey's multiple comparisons was used (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).