Figure 2.

Immune profiling prior to Mtb infection. Sixty days after vaccination with BCG or BCGΔBCG1419c mice were assessed for: (A) Number of viable BCG in inguinal LN, spleen and lung; (B) Number of total CD3⁺, CD3⁺CD4⁺, CD3⁺CD8⁺, CD3⁺CD4⁻CD8⁻ (DN) and CD3⁻NKp46⁺ cells (left plots), as well as numbers of total CD44⁺, CD44⁺CD69⁺CD103⁺ cells amongst CD3⁺CD4⁺ and CD3⁺CD8⁺ cells (right plots) in BALF and lung (C) lung immune cell infiltration; and (D) serum cytokines/chemokines. Results are presented as individual data points (A,C), pooled data means ± SEM (B), representative images (C) and as a heat map showing fold upregulation relative to naive controls (D) from two pooled independent experiments (n = 8–10) mice per group. Statistical analyses: One-way ANOVA per cell type followed by Dunnett's multiple comparisons test; significant differences relative to unvaccinated mice are indicated by asterisks: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Original magnification H&E × 25. BCG, Bacille Calmette–Guérin; s.c., subcutaneous; i.t., intratracheal; BALF, bronchioalveolar lavage fluid; LN, lymph node.