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. 2019 Mar 21;10:551. doi: 10.3389/fmicb.2019.00551

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Copyright © 2019 Chi, Zhang, Sun, Duan, Qiao, Luan and Lu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Development of a theophylline-responsive glgC-expression system in Synechococcus elongatus PCC7942. (A) Schematic representation of glycogen metabolism in cyanobacteria. GlgP, glycogen phosphorylase; GlgC, glucose-1-phosphate adenylyltransferase or ADP-glucose pyrophosphorylase; GlgA, glycogen synthase; G-1-P, glucose-1-phosphate; G-6-P, glucose-6-phosphate; ADP-G, ADP-glucose; PPP, pentose phosphate pathway; EMP, Embden-Meyerhof-Parnas pathway, glycolytic pathway; CBB cycle, Calvin-Benson-Bassham cycle; Pi, inorganic phosphate; PiPi, pyrophosphate. (B) Construction strategy of a theophylline-responsive riboswitch control system on glgC expression in PCC7942. KmR, kanamycin resistance gene; PglgC, native promoter sequence of glgC. (C) Genotype identification of the PCC7942-XC1 and PCC7942-WT by PCR.