Table 1.

Host range infection and efficiency of plating (EOP) of the phages Escherichia virus myPSH2311, Klebsiella virus myPSH1235, and Enterobacter virus myPSH1140.

Bacteria	Spot test (%)	High (EOP ≥ 0.5)	Moderate (EOP > 0.1- < 0.5)	Low (EOP ≤ 0.1)	No activity (EOP < 0.001)	Sum of EOP values
Escherichia virus myPSH2311 lytic activity against different pathotypes of E. coli
EPEC (n = 12)	10 (83%)	5	1	1	3	5.30
EHEC (n = 10)	6 (60%)	2	0	1	3	1.91
ETEC (n = 8)	4 (50%)	3	0	0	1	3.10
EIEC (n = 11)	9 (82%)	5	3	0	1	5.55
EAEC (n = 14)	10 (71%)	6	0	2	2	5.86
UPEC (n = 21)	17 (81%)	11	0	1	5	10.75
Unknown (n = 4)	2 (50%)	2	0	0	0	1.60
Total (n = 80)	58 (73%)	34	4	5	15	34.07
Klebsiella virus myPSH1235 lytic activity against different serotypes of K. pneumoniae
Serotype K1 (n = 3)	2 (67%)	1	1	0	0	1.20
Serotype K2 (n = 7)	5 (71%)	2	1	1	1	2.85
Serotype K5 (n = 9)	6 (67%)	2	0	2	2	2.10
Unknown (n = 25)	10 (40%)	5	1	1	3	5.85
Total (n = 44)	23 (52%)	10	3	4	6	12.0
Enterobacter virus myPSH1140 lytic activity against different species of Enterobacter
E. cloacae (n = 15)	15 (100%)	7	3	1	4	8.50
E. hormaechei (n = 4)	3 (75%)	2	0	0	1	2.72
E. asburiae (n = 4)	2 (50%)	2	0	0	0	2.10
E. aerogenes (n = 3)	2 (67%)	2	0	0	0	1.44

Values represent the lytic activity of the respective phage against the target bacterium. EPEC (enteropathogenic E. coli), EHEC (enterohemorrhagic E. coli), ETEC (enterotoxigenic E. coli), EIEC (enteroinvasive E. coli), EAEC (enteroaggregative E. coli), UPEC (uropathogenic E. coli). EOP was calculated using double agar overlay method. EOP was deemed as ‘High’ if the test bacterium had at least 50% activity (EOP ≥ 0.5) compared to the host bacterium, ‘Moderate’ if between >10 and <50% (EOP > 0.1- < 0.5), anything < 10% (EOP ≤ 0.1) was reported as ‘Low.’