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. 2019 Mar 21;10:316. doi: 10.3389/fpls.2019.00316

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Copyright © 2019 Palumbo, Vannozzi, Magon, Lucchin and Barcaccia.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression level of B-sister class genes VviABS1, VviABS2, and VviABS3. Preanthesis samples were collected 14 (stage I), 11 (stage II), 8 (stage III), 6 (stage IV), and 1 (stage V) days before flowering, whereas postanthesis samples were collected 6 (stage VI) and 8 (stage VII) days after flowering when fecundation had already occurred. The blue dotted line indicates full anthesis. A, anther; CA, cap; CL, calyx; E, embryo; F, filament; O, ovary. Transcript levels were normalized to the expression of UBIQUITIN gene and plotted as normalized transcript expression. Bars indicate the SE of three biological replicates each one composed of three technical replicates.