Figure 1.
GAP43 and SCG10 immunostaining and Western blotting show that ascorbic acid (AA) accelerates the regrowth of injured axons in the early stage after sciatic nerve crush injury. (A–C) Representative samples of GAP43-immunostained longitudinal sciatic nerve sections at 3 dpi from the sham group (A), saline group (B), and AA group (C). Asterisks represent the lesion site. (D,E) Western blots and schematic diagrams of the statistical analysis showing the protein level of GAP43 in the distal trunk of the injured sciatic nerves (n = 3, *P < 0.05). (F,G) Representative samples of SCG10-immunostained longitudinal sciatic nerve sections from the sham group (F), saline group (G), and AA group (H). Asterisks represent the lesion site. (I,J) Western blots and schematic diagrams of the statistical analysis showing the protein level of SCG10 in the distal trunk of the injured sciatic nerves (n = 3 for each test, *P < 0.05).