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. 2019 Mar 27;9:5243. doi: 10.1038/s41598-019-41649-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Alignment of nucleotide sequences of exon 16 3′-end and downstream intron across nine species. Accession numbers of different species are: camel (NCBI Gene ID: 105090951), pig (NCBI Gene ID: 445515), donkey (NCBI Gene ID: 106835119), horse (NCBI Gene ID: 100327035), rabbit (NCBI Gene ID: 100009288), bovine (NCBI Gene ID: 282209), goat (NCBI Gene ID: 100861229), sheep (NCBI Gene ID: 443383), and buffalo (NCBI Gene ID: 102395699). Exon sequences are in bold, intron sequences are in italics. Perfectly conserved nucleotides are dark-grey shaded. Nucleotides identical in more than eight animal species are light-grey shaded. Dashes in ruminants indicate missing nucleotides that are highlighted in yellow in the other species. The dinucleotide GT, highlighted in green in the camel sequence, generates the preferential site of splicing occurring within exon 16 that leads to the main α_s2-CN isoform first described by Kappeler et al.²².