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. 2019 Mar 27;9:5281. doi: 10.1038/s41598-019-41717-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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FOXS1 regulates gastric cancer cell proliferation and the ability of colony formation. (A) The knockdown effect of siRNA targeting at five sites (137, 212, 275, 641 and 1040) of FOXS1 mRNA at mRNA levels were detected by RT-PCR. (B) The knockdown effect of siRNA targeting at two sites (137 and 641) of FOXS1 mRNA at protein levels were detected by WB. (C) The effect of FOXS1 knockdown by transfection with siRNA-137 or siRNA-641 targeting FOXS1 on cell viability was determined by Cell Proliferation Assay kits. (D) The effect of FOXS1 knockdown by transfection with siRNA-137 targeting FOXS1 on cell colony formation was analyzed by Crystal violet staining, and quantitative analysis of cell colony numbers was showed in the right panel. (E) The effect of FOXS1 knockdown on cell cycle distribution. (F,G) Overexpression effect of FOXS1 at mRNA levels was confirmed by RT-PCR and at protein levels was confirmed by WB. (H) Effect of FOXS1 overexpression on cell viability were detected by Cell Proliferation Assay kits. (I) The effect of FOXS1 overexpression on cell colony formation was analyzed by Crystal violet staining, and quantitative analysis of cell colony numbers was showed in the right panel. (J) The effect of FOXS1 overexpression on cell cycle distribution.