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. 2019 Mar 27;9:5281. doi: 10.1038/s41598-019-41717-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The analysis and identification of FOXS1 core promoter. (A) Schematic diagram of FOXS1 upstream 1100 bp promoter reporter constructs. (B) Dual luciferase reporter assays were used to determine the core promoter activity region of FOXS1. (C,D) Prediction of transcription factors binding sites in the FOXS1promoter region using TFBSs, JASPAR and PROMO. (E,F) The influence of transcription factors on FOXS1 promoters were determined by dual luciferase reporter assays. (G) The expression of NFKB1 and FOXS1 after NFKB1 overexpression was determined by RT-PCR in MKN45 cells. (H) The expression of NFKB1 and FOXS1 after NFKB1 overexpression was determined by western blot in MKN45 cells. GAPDH was internal control. (I) The effect of transcription factors NFKB1 on FOXS1 promoters with NFKB-binding mutated sites were determined by dual luciferase reporter assays. (I) ChIP assays were performed to detect NFKB1 directly bound in the FOXS1 promoter region in HGC-27 cells.